A Secret Weapon For flow cytometers
A Secret Weapon For flow cytometers
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At the moment, the early signs had been dismissed as debris, though the retrospective analysis shown shared attributes Along with the problematic cells in lifestyle. Tracing the an infection aided the lab set up additional laboratory strategies for screening and safety of assay-vital mobile lines.
Regardless of whether you’re a starter or an authority, explore our sources to advance your study and experience in flow cytometry.
Combine acoustic focusing technological innovation with large-parameter Examination for adaptable spectral unmixing and traditional workflows.
Discover new insights into your experimental details by visualizing populations employing combined fluorescence and morphology knowledge. Our program interface wizards help downsampling, concatenation (merged data files) and dimensionality tools which have been designed in to the software for an intuitive, streamlined information analysis encounter.
"An image isn't quite truly worth 1000 dots, but it really genuinely helps to see pictures. It is great to check out pictures that correlate with staining. Such as, AnnexinV-beneficial cells that happen to be morphologically various than balanced cells, or CD14+ monocytes which have been larger plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Furthermore, it's illuminating to check out how many doublets slip by way of classic singlet gates, and great to confirm the position of unpredicted double-constructive events in my Investigation."
Pair your Attune flow cytometer having an autosampler for elevated workflow performance, and increase robotic integration for maximum throughput.
Our experts will structure panels for yourself as persistently you may need—free of charge, no products order required.
The Attune NxT cytometer is the trusted workhorse instrument your lab can rely upon for reliable and trusted effectiveness. The Attune CytPix model includes a higher-pace brightfield digicam and AI-driven graphic Evaluation software, with the latest upgrades which includes machine Finding out tools and an intuitive consumer interface, enabling Improved details precision, accuracy, and quicker insights into sample biology.
People can watch occasion visuals in actual-time to substantiate sample good quality, enhance protocols, and history the morphology of each and every mobile inhabitants identified for Examination. Imaging seize also Rewards from acoustic concentrating, which helps to place the cells in order that a sharp, centered graphic is acquired.
Seem waves empower precise mobile alignment, resulting in Extraordinary info high quality and ~10X speedier sample flow charges.
We previously shown the strength of imaging Auto-T/Ramos cell interactions. Allow’s evaluate just the populace of biggest interest, the double positive gatherings, To find out more. We are able to now use prolonged morphological parameters (in this article, circularity and skewness of intensity) to even further study the capabilities of such populations and refine gating on these events, growing facts robustness.
Crafted-in dimensionality reduction resources greater resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to confirm expected populace resolution in lysed full human blood. Consumer can check out functions from Each and every cluster for visual affirmation of mobile phenotypes.
Versatile payment plan from Agilent Economic Options assist you to obtain the vital instrumentation you require for speedy venture, or to increase your lab.
At the heart of each and every Attune Flow Cytometer lies our innovative acoustic concentrating technology, which makes use of seem waves to meticulously align cells within the flow mobile. This impressive method surpasses regular hydrodynamic techniques, causing faster processing speeds, Remarkable information excellent, and amplified sample throughput.
Because the cell line experienced Formerly been analyzed within the Attune CytPix Flow Cytometer, the scientists went back again to the photographs and have been in a position to document the microbial an infection a minimum of five days previously.
Agilent Worth Assure guarantees you at least ten years of instrument use through the day you purchase. If not, We'll credit score you Using the residual here value of the method toward an upgraded design.
In this article, we clearly show that by gating techniques using impression-based mostly quantitative parameters can distinguish interacting cells from coincident gatherings to additional accurately review interacting cells.